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Effect of temperature shock on soybean microspore embryogenesis BABT
Moraes,Ana Paula de; Bonadese-Zanettini,Maria Helena; Callegari-Jacques,Sídia Maria; Kaltchuk-Santos,Eliane.
In this study the effect of cold and heat shock on androgenetic induction in soybean anther culture was tested. Anthers of soybean were subjected to 4 and 33ºC, while the anthers used for control were maintained at 25ºC. Cytological analysis done during the 30 days of culture showed that frequencies of symmetrical binucleated and multicellular pollen grain did not differ among temperature treatments. Multicellular pollen grain could be formed by symmetrical division, as well as by assymetrical division. In relation to the embryo induction, the results of the treatments did not differ too. These results suggested that these treatments did not induce a sporophytic pathway in soybean.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anther culture; Soybean; Androgenesis; Symmetrical mitosis; Temperature shock; Stress.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000400006
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Proline content and protein patterns in Eucalyptus grandis shoots submitted to high and low temperature shocks BABT
Souza,Gustavo M.; Cardoso,Victor J. M.; Gonçalves,Antonio N..
Proline content and protein patterns changes in response to temperature shocks of both acclimated and non acclimated E. grandis shoots cultivated in vitro were investigated. Analysis of soluble proteins through SDS-PAGE and proline were carried out after 12h at 12ºC (cold acclimation) or 33ºC (heat acclimation), and immediately after temperature shocks at 41ºC and 0ºC. Analyses were also performed 24h after the temperature shocks (recovery period). Temperature treatment at 0ºC did not change soluble protein patterns both in acclimatized and non acclimatized plants, whereas cold temperature induced high proline levels, which kept relatively high after recovery period. Three novel, probably HSPs, proteins (90.5, 75 and 39 kDa) were observed in both...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heat shock protein; Proline; Recovery; Stress; Temperature shock.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300004
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